Sartorious Octet® R4 Protein Analysis System

Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking in the biosensor tray

Data Presentation

• Graphs displaying real-time kinetic binding sensorgrams, fitted result plots, and residuals of fits

• Concentration data analysis including calibration curves and output of tabulated concentration data

• Tabulated kinetic data

• Epitope binning and cross-blocking matrices and trace overlays

Sample Type

Proteins, antibodies, peptides, DNA, RNA, liposomes, viruses and VLPs in various media including serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates

Number of Spectrometers

4

Maximum Simultaneous Reads

4

Data Collection Rate

2, 5 or 10 Hz

Sample Position and Format

1 standard 96-well, black, flat-bottom microplate

Sample Volume

Orbital Flow Capacity

Static or 100–1500 rpm

Analysis Temperature Range

15°C to 40°C, in 1°C increments

Quantitation and Kinetics

Throughput

Up to 4 assays in parallel

Molecular Weight Detection

>150 Da

Analysis Time per Sample

Real-time kinetic binding experiments from 5 minutes to 4 hours

1 mM – 10 pM

Baseline Noise (RMS)

<3.0 pm

Baseline Drift

≤ 0.12 nm/hour

Quantitation Precision

CV <10%